JW steel EN10268 St37-2G steel thickness

Incubate in a shaking incubator at 37 C and 225 rpm. 3. Later in the day: Complement the 250 mL minimal salts medium with 2 mM MgSO4 (0.5 mL of 1 M MgSO4 stock solution), 1% (v/v) 100 trace metal solution (2.5 mL), 2% (v/v) glucose (5 mL of 20% (v/v) glucose stock solution), 50 mg/L thiamine (250 μL of 50 mg/mL thiamine stock